RNA sequencing

RNA sequencing

RNA Sequencing

RNA sequencing is a powerful method for qualitative and quantitative analysis of the human transcriptome and regulatory RNAs.

Transcriptomics is used for differential gene expression, detection of alternative splicing and characterization of rare or novel transcripts.

Vascular Genetix offers comprehensive human, non-human and metatranscriptomic RNA Sequencing Services for different diagnostic or scientific purposes.

GEx Specifications

Sample: ≥20 μl, ≥25 ng/μl High or Low quality RNA

Library preparation: QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen)

Inclusions (Sequencing, Bioinformatics, Raw data transfer): GEx analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports);
free data transfer of FastQ, Bam files and Reports

Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 1x≥50 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 80%

Quality control: Full quality assurance and guaranteed specifications

Turnaround time: min. 2 – max. 4 weeks

mRNA-Seq Specifications

Sample: ≥20 μl, ≥50 ng/μl High quality RNA (RIN>7)

Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer)

Inclusions (Sequencing, Bioinformatics, Raw data transfer): mRNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports

Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 2x≥75 bp paired-end run; Seq throughput: 50/100 M PE read per sample; Q30 ≥ 80%

Quality control: Full quality assurance and guaranteed specifications

Turnaround time: min. 2 – max. 4 weeks

RNA-Seq Specifications

Sample: ≥20 μl, ≥50 ng/μl High or Low quality RNA

Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with RiboNaut rRNA Depletion HMR (PerkinElmer)

Inclusions (Sequencing, Bioinformatics, Raw data transfer): RNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports

Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 2x≥75 bp paired-end run; Seq throughput: 100/200 M PE read per sample; Q30 ≥ 80%

Quality control: Full quality assurance and guaranteed specifications

Turnaround time: min. 2 – max. 4 weeks

Small RNA-Seq Specifications

Sample: ≥20 μl, ≥50 ng/μl High quality RNA

Library preparation: Nextflex Small RNA-Seq Kit v4 (PerkinElmer)

Inclusions (Sequencing, Bioinformatics, Raw data transfer): Small RNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports

Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 2x≥40 bp paired-end run; Seq throughput: 20/40 M PE read per sample; Q30 ≥ 85%

Quality control: Full quality assurance and guaranteed specifications

Turnaround time: min. 2 – max. 4 weeks