RNA sequencing
RNA Sequencing
RNA sequencing is a powerful method for qualitative and quantitative analysis of the human transcriptome and regulatory RNAs.
Transcriptomics is used for differential gene expression, detection of alternative splicing and characterization of rare or novel transcripts.
Vascular Genetix offers comprehensive human, non-human and metatranscriptomic RNA Sequencing Services for different diagnostic or scientific purposes.
- Gene Expression Profiling (GEx): determines the Gene-level abundance and differential expression of coding transcripts
- mRNA-Seq: targeting poly(A) RNAs provides a sensitive option for detection and quantitation of even rare coding transcript variants
- RNA-Seq: RNA samples are enriched for both mRNA and non-coding RNAs to provide a comprehensive view of the whole transcriptome
- Small RNA-Seq: targets small regulatory RNAs such as miRNA for profiling and discovery.
GEx Specifications
Sample: ≥20 μl, ≥25 ng/μl High or Low quality RNA
Library preparation: QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): GEx analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports);
free data transfer of FastQ, Bam files and Reports
Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 1x≥50 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 80%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks
mRNA-Seq Specifications
Sample: ≥20 μl, ≥50 ng/μl High quality RNA (RIN>7)
Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): mRNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports
Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 2x≥75 bp paired-end run; Seq throughput: 50/100 M PE read per sample; Q30 ≥ 80%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks
RNA-Seq Specifications
Sample: ≥20 μl, ≥50 ng/μl High or Low quality RNA
Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with RiboNaut rRNA Depletion HMR (PerkinElmer)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): RNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports
Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 2x≥75 bp paired-end run; Seq throughput: 100/200 M PE read per sample; Q30 ≥ 80%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks
Small RNA-Seq Specifications
Sample: ≥20 μl, ≥50 ng/μl High quality RNA
Library preparation: Nextflex Small RNA-Seq Kit v4 (PerkinElmer)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): Small RNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports
Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 2x≥40 bp paired-end run; Seq throughput: 20/40 M PE read per sample; Q30 ≥ 85%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks